Thu11272014

Last updateWed, 22 Oct 2014 9am

Southampton/Portsmouth

Location of laboratory

Southampton
Southampton General Hospital, Southampton, Hampshire

Portsmouth
Department of Clinical Microbiology (HPA Collaborating Laboratory)

Name and postal address of the laboratory

Southampton
Health Protection Agency, Southeast Region Laboratory,
Department of Microbiology and Virology
South Block
Southampton General Hospital
Tremona Road, Southampton SO16 6YD

Portsmouth
Pathology Centre
Queen Alexandra Hospital
Cosham, Portsmouth, PO6 3LY

DX address Southampton
HPA Southeast
DX 6880300
Southampton 90 SO

Portsmouth
Portsmouth Microbiology Laboratory
DX 6882700
Cosham (Portsmouth) 91 PO

Telephone number for enquiries & fax number  Southampton
Tel: 023 8079 6408
Fax: 023 8070 2530

Portsmouth
Tel: 02392 286201
Fax: 02392 388395
Staff

Dr. Emanuela Pelosi
Emanuela.Pelosi@uhs.nhs.uk 
Tel: 02380 795101
Consultant Virologist

 

Dr.Gill Underhill
gill.underhill@porthosp.nhs.uk 
Tel: 02392 286886
Consultant Virologist

Dr. Peter Marsh
Peter.Marsh@uhs.nhs.uk 
Tel: 02380 794238
Clinical Scientist

Dr. Steve Green
Steve.Green@uhs.nhs.uk 
Tel: 02380 794810
Clinical Scientist

Dr. Simon Friar
Simon.Friar@uhs.nhs.uk 
Tel: 02380 798760
Clinical Scientist

Mrs. Susan Mason
Sue.Mason@uhs.nhs.uk 
Tel: 02380 795214
Lead Biomedical Scientist/laboratory manager


Lynn Collins
lynn.collins@porthosp.nhs.uk 
Tel: 02392 286201
Operational Manager (virology)

Miss. Emma Andrews
Emma.Andrews@uhs.nhs.uk 
Tel:02380 798760

Senior Biomedical Scientist (Molecular)

Mr. Christopher Labdon
Christopher.Labdon@uhs.nhs.uk 
Tel: 02380 796342
Senior Biomedical Scientist (Serology)

Allyson Lloyd
Allyson.lloyd@porthosp.nhs.uk 
Tel: 02392 286201
Senior Biomedical Scientist

William Rivenburg

William.Rivenburg@uhs.nhs.uk

Biomedical Scientist
What do we do? Diagnostic Assays

Molecular diagnosis

 

In Southampton the molecular diagnostics laboratory uses real time PCR (ABI 7500, RotorgeneQ, Lightcycler 480, Lightcycler 2.0,) for the detection of viral and bacterial nucleic acids in clinical samples. Tests are routinely performed for the detection of the following pathogens: HSV, VZV, CMV, EBV, Adenoviruses, Meningococci, Enteroviruses,C.trachomatis (Roche and Abbott platforms) and Neisseria gonorrhoeae (Abbott platform) as well as respiratory viruses (see below) and noroviruses.

 

Quantitative assays are available for HCV RNA, HBV DNA, HIV RNA (Abbott M2000) CMV DNA and EBV DNA (in-house assay). HCV genotyping (Abbott M2000) is also available.

Norovirus RNA testing of stool samples is carried out for the Southeast region

 Routine respiratory PCR panel includes:

Influenza A virus, Influenza B virus, RSV subgroup A and B, Parainfluenza 1, 2 and 3, Human metapneumovirus and Adenovirus

Influenza A can be subtyped (H1, H3, H5) and also oseltamivir resistance testing is available for H1N1pdm09

Pneumocystis jirovecii PCR is also available

In Portsmouth, quantitative assays for Hepatitis C PCR and HIV viral load are performed using the Roche Cobas AmpliPrep and Taqman platforms. The Becton Dickinson Viper with magnetic extraction uses a Strand Displacement Assay to detect Chlamydia trachomatis and Neisseria gonorrhoeae.

The molecular section routinely performs diagnostic testing for HSV, VZV, EBV, CMV, Adenovirus, Enterovirus,Norovirus, Influenza A and B, and specific H1N1 on the Roche LightCycler 2.0.

In addition, blood samples from patients with, or at risk of, thrombophilia are examined for Factor V Leiden and Factor II prothrombin using the Roche LightCycler 2.0.

Qiagen LipuiChip 200 is available for the following respiratory viruses (using Artus Resplex kit) Influenza A and B, RSV A and B, Parainfluenza (4 types), Human metapneumoviruses A and B, Coxsackieviruses/Echovirus, Rhinovirus, Adenovirus, Coronavirus and Bocavirus.

 

Serology

Both laboratories offer an extensive range of screening and diagnostic serology tests.


R&D

Clinical Scientists Dr. Peter Marsh and Dr. Steve Green have undertaken a number of collaborative projects with different clinical research groups and academic partners both at Southampton University Hospitals Trust (SUHT) and at the University of Southampton; additionally they also work together with Kings College London and CEPR. In particular, Dr. Marsh has established an important collaborative relationship with Professor Ian Clarke of The University of Southampton, currently including an MRC-funded project for the development of high resolution genotyping of Chlamydia trachomatis, leading to detailed studies of sexual networks.

 

Dr Marsh and Dr Green are also involved in the supervision of BMS M.Sc. projects, of which there have been successful outcomes resulting in the development and validation of several molecular diagnostic assays (see above).


Out of hours’ service

Out of hours HIV testing service on site for pregnant women in labour.

Full out of hours organ donor and recipient screening service for the Wessex renal transplant unit. 
Links

This Page was updated On 09/07/2013